ABSTRACT
OBJECTIVE: To investigate the effects of puerarin on impaired endothelium-dependent relaxation induced by glycosylated bovine serum albumin (GBSA) in rabbit thoracic aorta and its mechanisms. METHODS: The rings were incubated with GBSA for 30 min to induce endothelial dysfunction, and with puerarin(0.25, 0.5 and 1 g · L-1), A-nitro-L-arginine methyl ester (L-NAME, 30 νmol · L-1), and indomethacin(10 μmol · L-1) to investigate the protective effect of puerarin on impaired vascular endothelial function elicited by GBSA, and the effect of L-NAME and indomethacin on the protective effect of puerarin. Moreover, the content of nitric oxide (NO) and malonaldehyde (MDA) and the activity of superoxide dismutase(SOD) in the rings were measured. RESULTS: Exposure of aortic rings to GBSA (200 mg · L-1) for 30 min resulted in a significant inhibition of endothelium-dependent relaxation, but had no affect on endothelium-independent relaxation. GBSA significantly decreased the level of NO and the activity of SOD but hugely increased the content of MDA in vascular tissues. Pre-incubation of aortic rings with puerarin markedly attenuated the inhibition of endothelium-dependent relaxation induced by GBSA. This protective effect of puerarin (1 g · L-1) was partially inhibited by L-NAME but not by indomethacin. Puerarin obviously increased NO level and SOD activity but evidently decreased MDA content in rings. CONCLUSION: Puerarin can protect against vascular endothelial dysfunction caused by GBSA and its mechanisms may be related to enhancing NO synthesis and its anti-oxidation.
ABSTRACT
ObjectiveTo assess the satisfaction of general practical students' perception with the two modes of histologic laboratory instruction,and obtain the advantages and disadvantages of each mode.MethodsWeb-based database was established and in the questionnaires were delivered to the general practical students ( n=193 ).Results75％ of students agreed that the case study component of the webbased laboratory instruction was arranged in order.82％ of students identified with the detailed word explanation attached to every image presented.ConclusionStudents support the progression of web-based histologic laboratory instruction,but it is difficult to replace traditional microscope with web-based database in histologic laboratory instruction.